Original Source:    https://laboratoryquipment.blogspot.com/2020/02/facts-and-knowledge-about-vacuum-glove.html


The Vacuum Glove Boxes are a combination of the vacuum chamber and glove box. Vacuum Glove Boxes are made from 1 thick clear acrylic walls for making the Vacuum Glove Boxes water-tight/air-tight seal and capable of handling b29.8 in. Hg negative pressure. An inactive atmosphere is created when the vacuum pump removes the gases from the major chamber and replaced with argon or nitrogen gas. The chemicals should be dry before being placed in a glove box. For performing experiments in a non-combustible environment and low pressure which also requires the user to manipulate objects or non-harmful substances, the researchers should use Vacuum Glove Boxes.

Where are Vacuum Glove Boxes utilized?

Mostly Vacuum Glove Boxes are used for the manufacturing of semiconductor components, spacecraft engineering, lithium battery development and constructing a simulated vacuum environment.

How to use a Glove Box?

  1. Transfer of objects into the Glovebox:


  • Both doors should be closed. Turn the valve to fill and fill nitrogen in the chamber when it’s under vacuum. Make sure that both the doors are closed.
  • Transfer the object from the outer door in the chamber. For making it easier to reach it from inside the Glovebox the object should be slide as deep as possible. After this shut the door which is isolating the antechamber from the environment. Make sure that the door is not over tighten.
  • After closing the doors, evacuate the chamber by turning the valve and wait for the pressure to be at an acceptable vacuum level. Turn the valve to refill and then fill the chamber with nitrogen. Keep repeating this cycle till it is at least done 3 times.
  • Now the inner door to the glove box is opened. Place the object in a safe location in the glove box after removing it from the antechamber. Close the door to the antechamber.


  1. Transfer of the Objects from the Glovebox


  • The antechamber should be filled with nitrogen. Transfer the object in the chamber by opening the inner door and then the inner door to the antechamber should be closed. Make sure that if the object is containing chemicals it is properly sealed.
  • Remove the object from the outer door. The object should always be removed from the outer door.
  • Turn the valve to evacuate after closing the outer door. Make sure that you have closed the outer door before turning the valve to evacuate.

General Tips on Chemicals, Apparatus, Solvents and Waste Disposal

The apparatuses should by dry for entering into the glovebox. Only dry and deoxygenated chemicals should be taken in the glove box. Only the required amount of chemicals should be taken.20-ml vials are to be used for the transfer of solids and non-volatile liquids. Lab wipes and a rubber band should be used for wrapping and holding the top of the vials. Teflon stoppered flasks should be used for transferring volatile liquids. After finishing your work clean the area after taking out all the apparatuses. Use the Teflon-stoppered round-bottom flasks while taking in solvents. Use only dry deoxygenated solvents from the solvent stills. Every time the solvent bottles stored in the box should be capped. The gloves can be damaged from the solvent vapors. Lab wipes should be used for wiping the solvent spills. The waste should be disposed of in properly labelled waste beakers. Before disposing of the needles after use it is necessary to recap them. Replace the waste container in the glove box after disposing of the waste container in the lab waste. The waste should be disposed of in the misc. waste after cleaning up the spills on the glove box. It is important that the liquid waste bottle should be capped every time. Your hands from the gloves should not be removed or inserted quickly. If you will quickly remove or insert your hands from the gloves the ambient air from the external environment will enter through any micropores on the gloves. The atmosphere in the box will get compromised if the needles and blades are not carefully used because the gloves are extremely delicate. The chambers should always be left under vacuum after use. Always keep the record of the activity and maintenance performed on the glovebox. Maintenance of regeneration of purification system, vacuum pump oil change, etc. should be performed on a regular schedule.


The information given above explains the meaning of Vacuum Glove Boxes, how to use a  Portable Glove Boxes,  transfer of the objects from the glove box and general tips on chemicals, apparatus, solvents, and waste disposal. The above information provides you the details of the glove box.